Is hybridoma production about to take a quantum leap forward?

This obstacle has been effectively removed by Neowater®, a novel nanoparticle-doped (NPD) water created by a unique patented water-based nanotechnology.

Neowater® is a non-toxic form of water that mimics intracellular water, which is found uniquely in the human body and its cells. NPD water is characterized by a shifting in the physical properties of ordinary water, imparting new levels of compatibility with hydrophobic substances. Neowater® also maintains the beneficial effect of RF radiation on water for years after its production, thanks to its extraordinary structural stability.

The researchers performed numerous experiments to test the growth rate of hybridoma cells in NPD-based media. They specifically tested the effect of the NPD environment on the complete process of human monoclonal antibody production, and their results were published recently in the BMC Biotechnology Journal.

To evaluate the hybridoma formation process (utilizing the chemical fusion method), the researchers received samples of human peripheral blood mononuclear cells (PBMC) from several donors; each sample was tested either in a NPD or a DI (de-ionized) environment. In referring to the results, the researchers stated: “We witnessed a statistically significant difference in the yield of hybridoma cells between NPD and DI environments.”

In another experiment, the isolation of subclones and autocrine activity of hybridoma cells was tested. The researchers reported: “We observed greater clonal outgrowth of antibody-secreting hybridoma cells in NPD-based media as compared to DI-based media.” Moreover, they found that “the cloneability of cells from a semi-stable clone is also enhanced in NPD-based media.”

The researchers noted that hybridoma clones grown on NPD-based media secreted more monoclonal antibodies into their environment. However, they also observed that “Some cells grow faster in NPD-based media…This result might not reflect greater secretion per cell, but rather greater proliferation of cells with a similar secretion.” After normalization of this biased situation, the researchers calculated that “the secretion of monoclonal antibody in NPD-based media is roughly twice that obtained in DI-based media.”

This interesting and unexpected result led the researchers to conduct further tests: To what extent are cell proliferation rates affected by NPD-based media" Growing CHO (Chinese Hamster Ovary) cells in NPD-based and DI-based media, the researchers observed an unmistakable increase in proliferation when cells were incubated in NPD-based media, in comparison to the DI-based media: "an increase by an average of nearly 30%” .